Tiny DNA Tweezers Catch and Release Objects on Demand

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PostPosted: Mon Aug 31, 2009 9:47 am    Post subject: Tiny DNA Tweezers Catch and Release Objects on Demand Reply with quote

Researchers in China are reporting development of a new DNA “tweezers” that are the first of their kind capable of grasping and releasing objects on-demand.

A 12% native PAGE run at pH 5.0 and viewed by dye staining and fluorescent imaging (based on emission from a labeled DNA target) to show that DAE tweezers can be effectively operated between “open” and “close” states and exert folding and unfolding actions on a captured DNA object: (a) “open” and “close” cycling of the tweezers; (b) gel stained by stains-all; (c) a fluorescent gel; and (d) superimposed gel images of (c) on (b). Note that the fluorescence image was taken prior to gel staining.

The microscopic tweezers could have several potential uses, the researchers note. Those include microsurgery, drug and gene delivery for gene therapy, and in the manufacturing of nano-sized circuits for futuristic electronics. Their study is scheduled for the November 12 issue of the weekly Journal of the American Chemical Society ( Catch and Release: DNA Tweezers that Can Capture, Hold, and Release an Object under Control".

Zhaoxiang Deng and colleagues note that other scientists have developed tweezers made of DNA, the double helix molecule and chemical blueprint of life. Those tweezers can open and close by responding to complementary chemical components found in DNA's backbone. However, getting the tweezers to grasp and release objects like real tweezers has remained a bioengineering challenge until now.

The scientists describe development of a pair of DNA tweezers composed of four DNA strands — three which act as the “arms.” In laboratory studies, the scientists showed that they could grab a piece of target DNA in the arms of the tweezers and release it on-demand using a controlled series of hydrogen bonding and pH changes. The scientists used fluorescent gel imaging to confirm the effectiveness of the tweezers' operation.

Source: http://portal.acs.org/portal/acs/corg/content?_nfpb=true&_pageLabel=PP_ARTICLEMAIN&node_id=223&content_id=WPCP_011130&use_sec=true&sec_url_var=region1#P60_5326
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