3D tissue models for studying bacterial infection
A research team, co-headed by Dr. Woo Lee and Dr. Hongjun Wang of Stevens Institute of Technology, has published a paper describing a new method that generates three-dimensional (3D) tissue models for studying bacterial infection of orthopedic implants. Dr. Joung-Hyun Lee of Stevens, and Dr. Jeffrey Kaplan of the New Jersey Dental School, are co-authors of the research. Their paper, appearing in the journal Tissue Engineering, demonstrates a physiologically relevant approach for studying infection prevention strategies and emulating antibiotic delivery using 3D bone tissues cultured in microfluidic devices.
With over 1 million hip and knee replacement procedures being performed in the United States every year, orthopedic implants have become relatively common. Despite advances in implant design, hospitals have been unable to address bacterial infection, the leading cause of failure in orthopedic implants. A significant barrier to successfully developing infection-fighting drugs or biomaterials has been the inadequacy of laboratory equipment to create clinically relevant environment with traditional in vitro methods.
The researchers seeded 0.02 mL microfluidic channels with osteoblasts and inoculated the channels with Staphylococcus epidermis bacteria, a common pathogen in orthopedic infections. Nutrient solutions were pumped through the channels at a concentration and flow rate mimicking conditions within the human body. Bone tissue cells and bacteria within the channels were imaged with a microscope and effluent was analyzed for bacteria count.
Microfluidic devices, together with finely-tuned dynamic flow settings, have the potential to provide realistic bone tissue models in clinical scenarios. As opposed to the static 2D Petri dish surfaces, microfluidic channels present a realistic environment for cells to grow and adhere in three dimensions. Dynamic fluid motion through the channels—with solutions potentially carrying antibiotics or other novel drugs—further mimics real-world conditions previously unrealizable in a lab setting.
The research team is comprised of Dr. Woo Lee, George Meade Bond Professor in Chemical Engineering and Materials Science; Dr. Hongjun Wang, Assistant Professor of Biomedical Engineering; Dr. Joung-Hyun Lee, Research Associate and 2010 Ph.D. graduate of Stevens; and Dr. Jeffrey Kaplan, Associate Professor in the Department of Oral Biology at the New Jersey Dental School. Dr. Joung-Hyun Lee, as the first author of this paper, used her background in microfabrication to discover the conditions for growing bone tissues in the microfluidic device channels while integrating capabilities in the laboratories of Lee, Wang, and Kaplan. This research was sponsored the Nanoscale Interdisciplinary Research Team program of the National Science Foundation (NSF). Also, Dr. Lee and Dr. Wang are principal investigators on a new grant from the NSF Biomaterials program, awarded earlier this year. In this new project, they plan to use the newly developed 3D tissue model to evaluate the efficacy of inkjet-printed infection-preventing biomaterials.
The researchers' published paper is a preliminary demonstration of dynamic microfluidic cell cultures and work continues in the lab to establish successful applications of the technology and processes. The article can be found online here.
For more information about this important research at Stevens Institute of Technology, The Innovation University, please contact Dr. Woo Lee.
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